Single cell analyses on the thymus in physiological and pathophysiological conditions

Type de poste
Niveau d'étude minimal
Dates
Durée du poste
Contrat renouvelable
Contrat renouvelable
Date de prise de fonction
Date de fin de validité de l'annonce
Localisation
Adresse

105 boulevard de l’hôpital
75013 Paris
France

Contacts
Rozen Le Panse
Email du/des contacts
rozen.lepanse@upmc.fr
Description

Project:
We are looking for a highly motivated Postdoc fellow or experimented research engineer for single-cell analyses. We are studying human thymus, focusing on stromal cells, in physiological and pathophysiological conditions such as autoimmune myasthenia gravis and thymoma.
This research project will use different single cell approaches: 1) On dissociated cells using the droplet-based single-cell technology from 10X Genomix to generate single cell cDNA libraries and investigate gene expression by RNA sequencing (scRNA-seq). 2) On thymic sections, with the Hyperion imaging system (Fluidigm). This technology combines the high-multiplex capabilities of mass cytometry with imaging using metal-tagged antibodies for the simultaneous analysis of up to 37 markers.
The combined approaches will provide the unique opportunity to explore thymic cell heterogeneity and will provide information about cell localization and cell-cell interactions.

Required skills:
Applicants should have academic degree and PhD in biology and bioinformatics skills for single-cell data analyses. Background in histopathology would be a plus.
Autonomous and rigorous with a critical mind.
Ability to work as a member of a team
Good communication skills and a good level in English

Location:
The research team is based in the Center of Research in Myology - Hospital La Pitiè-Salpêtrière in Paris (https://www.institut-myologie.org/en/recherche-2/centre-de-recherche-en…).
Application:
Review of applications begins immediately. The contract for the postdoctoral position would be for 1 year, renewable. The salary will depend on experience. To apply, please submit: i) a cover letter summarizing research interests and expertise ii) a Curriculum Vitae (including publications if any), and iii) the names and contact information for at least two references. Questions and application should be sent to Rozen Le Panse (rozen.lepanse@upmc.fr).

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