1, avenue Pierre Fabre
66650 Banyuls sur mer
Conception of multi-omics data analysis pipeline to infer gene regulation interaction networks in marine chordates
The vertebrate nervous system is a fascinatingly complex structure and understanding how it appeared during evolution remains an unresolved question. Vertebrates belong to the chordate clade together with tunicates (ex: ascidians), their sister group, and cephalochordates (i.e. amphioxus) that present similar but much simpler nervous systems. In our lab, at the marine station of Banyuls sur Mer, we are interested in understanding the Gene Regulatory Networks that drive central and peripheral nervous systems formation in animals of these two marine groups in order to better understand how the complexity observed in vertebrates appeared. We have generated in the European amphioxus species (Branchiostoma lanceolatum) and in two ascidian species (Ciona intestinalis and Phallusia mammillata) RNA-seq and ATAC-seq data at different stages of development and in embryos in which signalling pathways have been modified, as well as in explants of embryos. The aim of the internship project would be to integrate available multi-omics data to identify the networks of gene interactions that drive the processes of nervous systems formation in our models. These networks will then be compared between them and to the networks described in vertebrates in order to shed light on the evolution of nervous systems at the level of whole genome regulation.
[stage conventionné uniquement]
Keywords: regulatory networks, central nervous system evolution, ATAC-seq, RNA-seq, systems biology, marine animals, Gene ontology