Mots-Clés

Microbiote résistance aux antibiotiques métagénomique résistome

Description

The project is called BLA-IMPACT for Impact of beta-lactam exposure on intestinal phenotypically characterized beta-lactamases. It has been funded by the French Research Agency (ANR) in 2020. The idea is to analyse the evolution of the microbiota of healthy volunteers exposed to ceftriaxone, ceftazidime-avibactam or piperacillin-tazobactam, taking into account the baseline resistome (especially beta-lactamases). The missions of the post-doc will be to manage the Illumina and Nanopore sequencing data, to look for beta-lactamases encoding genes (using PCM, see https://www.nature.com/articles/s41564-018-0292-6) and actively collaborate to the biostatistical analyses. The project involves two other teams in IAME (BIPID and QEM) and the French National Reference Center for antibiotic resistance in Clermont-Ferrand (Prof. Richard Bonnet). The duration of the post-doc is 18 months, with possibilities to move on other projects after.

The summary is below: The intestinal microbiota is central in the antibiotic resistance phenomenon. Nonetheless, the impact of antibiotics on the intestinal microbiota remains poorly described, mainly because of the lack of phenotypic characterization of the resistance mechanisms of intestinal bacteria. Here, we propose to study the impact of various beta-lactams regimens (ceftriaxone, piperacillin-tazobactam and ceftazidime-avibactam, with or without a colon-delivered charcoal-based adsorbent) the intestinal microbiota of healthy volunteers, leveraging fecal antibiotic concentrations, state-of-the-art metagenomic analysis (combining short and long reads), phenotypic characterization, in vitro evolutionary models and longitudinal data analysis. Altogether, BLA-IMPACT will help in bridging phenotypic and metagenomic data that are mandatory to understand how the intestinal microbiota evolved under antibiotic exposure. Besides, you shall be involved in other microbiota-related projects we are running in the lab. You can visit our website here https://www.iame-research.center/