Stage M2
Stage · Stage M2 · 6 mois Bac+5 / Master DSIMB · Paris (France)
Mots-Clés
VHH, Molecular modeling, Molecular dynamics simulation & analysis, Sequence analysis & Gromacs, conformation analysis, python, structural classification
Description
S3D-VHH: Structure, Dynamics & CDR classification of VHH camel antibodies
Résumé: The internship is associated with the European FEDER project, led by A.G. de Brevern, named S3D-VHH. The internship will be led by Dr A.G. de Brevern (Paris) and Dr A. Nadaradjane (St Denis de la Réunion) and will be fully integrated into the European project. Antibodies (Abs) play a major key role in the immune system as they recognize and bind specifically to an antigen. Classical IgGs (3000 residues) have hypervariable loops named Complementarity Determining Regions (CDRs), directly implicated in the interaction with the epitope. VHHs are camelid specific Abs and are very interesting as they are short (less than 130 residues), with only 3 CDRs, they are easy to produce, highly stable. The structure of these hypervariable loop regions is highly challenging to analyse & predict. Understanding the structure & dynamics of the CDR regions will be highly useful in designing and engineering VHH as potential therapeutic agents. The aim of this internship project is to produce an appropriate analytical tool to classify VHH using its sequence and structure. The first goal of this internship is to set up a methodology (potentially web server) that will annotate, analyse & classify a given VHH using its CDR regions. And the second goal is to analyse the dynamics of these hypervariable loop regions (around 100 molecular dynamics simulations are already available). Results of the approach will be compared to state-of-the-art approaches like PyIgClassify.Candidature
Procédure : Envoyez-nous votre CV & une LM: alexandre.de-brevern@inserm.fr & aravindan.nadaradjane@univ-reunion.fr
Date limite : None
Contacts
Dr. Alexandre de Brevern & Dr. Arun Nadaradjane
alNOSPAMexandre.de-brevern@inserm.fr
Offre publiée le 19 octobre 2021, affichage jusqu'au 15 décembre 2021