M2 : Neuropeptides expression profiling during starvation in Crassostrea virginica.

 Stage · Stage M2  · 6 mois    Bac+5 / Master   Station Biologique de Roscoff / Plateforme ABiMS · ROSCOFF (France)

 Date de prise de poste : 1 janvier 2023

Mots-Clés

Genomics Oyster Bioinformatics RNAseq

Description

University:

ABiMS, FR2424, Station Biologique de Roscoff (http://abims.sb-roscoff.fr/) and the Marine Animal Disease Laboratory, School of Marine and Atmospheric Sciences, Stony Brook University, NY, USA) https://you.stonybrook.edu/madl/).

Intership Address:

Station Biologique de Roscoff, Place Georges Teissier, CS 90074, 29688 Roscoff, France

Supervisor (to be contacted for applying):

- Last and first name: Corre Erwan (Research engineer) and Allam Bassem (Professor)

- Email, phone number: corre@sb-roscoff.fr, Bassem.Allam@stonybrook.edu

 

Internship description (½ to 1 page):

The eastern oyster Crassostrea virginica is one of the most important bivalve species on the east coast of the United States both for their socio-economical value and environmental impact. Indeed, this filter feeder plays a critical role in clearing seawater and providing habitats to many marine species. Despite the large number of studies on C. virginica and most specifically on their physiology, the neuropeptidome and nervous system in general of this species has received little attention (Catapane et al., 2016). This lack of information on neuropeptides limits the comprehension of physiological processes and most particularly their regulation.

One of the most intriguing physiological processes in bivalves is their feeding mechanism. Indeed, they have developed a strikingly-efficient ability to select their food, preferentially ingesting nutrient-rich particles while rejecting poor quality ones in pseudofeces (Newell and Jordan, 1983), therefore optimizing energy gain (Bayne et al., 1993). Our previous research has shown that this ability is mediated by interactions between carbohydrates associated with the surface of microalgae and C-type lectins present in mucus covering the feeding organs (Pales Espinosa et al., 2010, Pales Espinosa and Allam, 2018). Interestingly, previous results suggest that the regulation of particle uptake and sorting may be operated via modulation of lectin expression, therefore providing suspension feeding bivalves with a flexible means to qualitatively and quantitatively control particle uptake to satisfy internal requirements. How such a molecular cascade is initiated remains unclear but it could be linked to the triggering of neurohormonal control systems (De Jong-Brink et al., 2001).

The aim of this Master project (M2) is to probe the molecular mechanisms that control food capture and sorting in the oyster Crassostrea virginica by identifying neuropeptides regulated in 3 organs (i.e., visceral ganglia, digestive gland and esophagus) in response to starvation (RNAseq analysis) and linking these changes to the expression of mucosal lectins in feeding organs (e.g., WGCNA). The working hypothesis is that the expression of mucosal lectins is triggered by signals emitted in neural ganglia. At the end of this internship, the candidate will graduate with a good understanding of bivalve physiology and a solid working knowledge of large-scale transcriptomic data analysis.

 

References

Catapane EJ, Nelson M, Adams T, Carroll MA. 2016. Innervation of gill lateral cells in the bivalve mollusc Crassostrea virginica affects cellular membrane potential and cilia activity. J Pharmacol Rep, 1(2).

Newell RIE, Jordan SJ. 1983. Preferential ingestion of organic material by the American oyster Crassostrea virginica. Mar Ecol Prog Ser 13, 47-53.

Bayne BL, Iglesias JIP, Hawkins AJS, Navarro E, Heral M, Deslous-Paoli JM. 1993. Feeding behaviour of the mussel, Mytilus edulis: responses to variations in quantity and organic content of the seston. J Mar Biol Assoc UK, 73(4), 813-829.

Pales Espinosa E, Hassan D, Ward JE, Shumway SE, Allam B. 2010. Role of epicellular molecules in the selection of particles by the blue mussel, Mytilus edulis. Biol Bull, 219(1):50-60.

Pales Espinosa E, Allam B. 2018. Reverse genetics demonstrate the role of mucosal C-type lectins in food particle selection in the oyster Crassostrea virginica. J Exp Biol. 15, 221(6):jeb174094.

de Jong-Brink M, ter Maat A, Tensen CP. 2001. NPY in invertebrates: molecular answers to altered functions during evolution. Peptides 22, 309-315.

Candidature

Procédure : send your candidature to : Corre Erwan (Research engineer) and Allam Bassem (Professor) corre@sb-roscoff.fr, Bassem.Allam@stonybrook.edu,

Date limite : 30 juin 2023

Contacts

Erwan CORRE

 coNOSPAMrre@sb-roscoff.fr

Offre publiée le 18 octobre 2022, affichage jusqu'au 30 juin 2023