Mots-Clés

Genomics DNA Replicaiton Genome Instability Single-Cell/Molecular Omics Cancer Evoluation Neurogenesis

Description

The hosting structure

Institut Curie Research Center
Institut Curie is a major player in the research and fight against cancer. It consists of a Hospital group and a Research Center
of more than 1000 employees with a strong international representativeness.
The objective of the Research Center is to develop basic research and to use the knowledge produced to improve the
diagnosis, prognosis, and therapeutics of cancers as part of the continuum between basic research and innovation serving
the patient.

Context
Laboratory
We are looking for motivated and productive post-doctoral fellows to join our team “Replication Program and Genome
Instability” at the Institut Curie, Paris, France. The team focuses on using cutting-edge high-throughput genomic approaches
and genome-wide data analyses to study the spatio-temporal replication program of the human genome and how
replication stress impacts on genome stability in normal and cancer cells, in population as well as at single molecule/cell
level.

The project
It is well known that DNA replication is a vital process in all organisms. At each cell division, the activation of over 30,000
replication origins in a coordinated manner is essential to ensure the duplication of >6 billion base pairs of the human
genome. During differentiation and development, this program must adapt to changes in chromatin organization and gene
transcription. Its deregulation can challenge genome stability, which is a leading cause of many diseases including cancer
and neurological disorders. To move the field forward, novel approaches to investigate DNA replication at the single
molecule/cell level are required. Leveraging on our expertise in high-throughput single molecule/cell data analysis, our
Impulscience project of the Fondation Bettencourt Schueller (https://www.fondationbs.org/en/life-
sciences/impulsciencer/discover-2022s-laureates) aims to develop original interdisciplinary methodologies by combining
cutting edge high-throughput single molecule/cell imaging/sequencing, mathematical/computational modelling and
bioinformatics analyses. With these, we will study the replication program in mammalian cell lines and in developmental
model organisms under normal and pathological conditions. This project will finally be able to decipher, at an unprecedented
depth, how the replication program links with genome instability in cancer and during neurogenesis.

Bibliographical references
1. Promonet A., et al. Nat. Commun. 11:3940 (2020)
2. Wang, W. et al. Mol. Cell, 81, 2975-2988.e6 (2021)
3. Gnan, S., et al. Nat. Commun., 13, 2329 (2022)
4. Emerson, Nature. 606:812–819 (2022)
5. Li J., et al. Cell, 186:98-111.e21 (2023)
6. Liu Y., et al. Nucleic Acids. Res., gkac1239 (2023)

Constraints and risks
- Short periods of travel
- Use of biological/chemical products
- Possibly working with mouse models

Candidate Profile
Training and Skills required:
- Both wet-lab and dry-lab postdoc positions are available.
- Applicant should hold, or in the process of completing, a PhD degree in biology, bioinformatics, or related areas.
- Solid computational/statistical skills or a solid background in molecule/cellular biology, and a strong interest in
genome biology.
- Experience with single-cell omics, linear tracing analysis, high-throughput imaging analysis, or neurogenesis (e.g.
Neural Stem Cell culture and differentiation, mouse or organoid model, etc.) is a plus.
- Language skills: very good English level and communication skills.

Abilities
- Ability to work independently, managerial abilities, aptitude for working in a team.
- The candidate should be highly motivated, curious and enthusiastic to work in a collaborative team.
All our opportunities are open to people with disabilities

Contract information
Type of contract: Fixed-term contract.
Starting date: As soon as possible
Duration: up to 4 years (renewable contract)
Working time: full time
Remuneration: according to the current grids
Benefits: Collective catering, reimbursement of transportation fees up to 70%, supplementary health insurance
Location of the position: Paris, 5eme

Contact
Please send your CV, letter of motivation and 3 references, to job-ref-c9ognue9pr@emploi.beetween.com
CVs to be processed on the go, therefore the position could be filled before the deadline for applications.
Deadline for application: 31/06/2024

Institut Curie is an inclusive, equal opportunity employer
and is dedicated to the highest standards of research integrity.