Mots-Clés
Transcriptome
imagining methods
Stem cells
data reconstruction
Description
About Cell-ID
The Cell-ID program focuses on uncovering the molecular mechanisms that govern cell fate determination during neural development and how their disruption contributes to childhood brain cancers.
Our goal is to design strategies for a cell-based interceptive medicine: targeting the earliest cellular and molecular events in cancer to prevent disease progression.
Led by CNRS and Inserm, in collaboration with major French institutions and international partners, Cell-ID integrates omics technologies, advanced imaging, and computational modeling to study genome function and 3D nuclear organization in neural tissues under normal and pathological conditions. By combining cutting-edge experimental tools with predictive models, the program aims to define and modulate cell trajectories during neural development.
About this project
Imaging transcriptome evolution in live cells
Edouard Bertrand and Florian Muller
Single cell sequencing has revealed that cells differentiate along well-defined trajectories in the transcriptomic space. However, how the transcriptome evolves in real-time is poorly understood. The goal of the PhD will be to develop imaging-based methods to better apprehend the dynamic regulation of the transcriptome. To this end, transcription will be imaged using a combination of live and fixed cell methods during the differentiation of stem cells. Transcription will be visualized at both the global and gene-specific levels and the data will then be used to reconstruct the trajectories of single cells in real time.