Société Française de Bioinformatique

Mots-Clés

bioinformatique structurale artificial intelligence HPC proteome

Description

Condensin is a ring-shaped protein complex that drives the condensation of mitotic
chromosomes in eukaryotic cells. Current data indicate that condensin shapes mitotic
chromosomes by translocating DNA through its ring structure, thereby extruding DNA-loops of
increasing size. What remains unknown, however, is how such a DNA-loop extrusion reaction
could take place in vivo, in the crowded context of chromatin. Inevitably, condensin must, in the
course of loop extrusion, encounter DNA bound factors that may impact on its residence time
and/or its activity, but very few condensin binding partners have been identified so far. To better
understand the integrated functioning of condensin we want to search in an unbiased manner
for its binding partners. With that aim in mind, we want to combine our experimental approach
with an in silico pull-down approach using AlphaFold to discover new protein-protein
interactions. The Master intern will systematically run Artificial Intelligence programs to discover
undetected interactions between the components of condensin and the whole yeast proteome.

The Master intern should have a keen interest in bioinformatics and programming in python.
Also important is the knowledge of Unix systems. She (he) will develop his work in the group of
Pascal Bernard and the DAMM team at the Laboratoire de Biologie et Modélisation de la Cellule
(LBMC).

https://www.ens-lyon.fr/LBMC/equipes/dynamics-and-control-of-biological-assemblies-and-macromolecular-machines
https://www.ens-lyon.fr/LBMC/equipes/architecture-et-dynamique-fonctionnelle-des-chromosomes